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iscript complementary dna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript complementary dna synthesis kit
    Iscript Complementary Dna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6686 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complementary+dna+cdna/IScript+C+DNA+Synthesis+Kit/pm42884080-156-9-14
    Average 99 stars, based on 6686 article reviews
    iscript complementary dna synthesis kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Single molecule analysis of CENP-A chromatin by high-speed atomic force microscopy
    Article Snippet: .. Complementary DNA (cDNA) samples were prepared using the iQ SYBR Green supermix (#170–8880; Biorad) following manufacturer’s protocol. ..

    Article Title: Single molecule analysis of CENP-A chromatin by high-speed atomic force microscopy
    Article Snippet: .. Complementary DNA (cDNA) samples were prepared using the iQ SYBR Green supermix (#170–8880; Biorad) following manufacturer’s protocol. ..

    Purification:

    Article Title: Curcuma longa L. extract exhibits anti-inflammatory and cytoprotective functions in the articular cartilage of monoiodoacetate-injected rats
    Article Snippet: The extracted RNA was assessed for ratio and concentration using a NanoDrop™ spectrophotometer. .. Thereafter, complementary DNA (cDNA) was synthesised from purified total RNA (500 ng/mL) using the iScript TM cDNA Synthesis Kit (Bio-Rad). .. Real-time polymerase chain reaction (PCR) was performed using a CFX Connect TM Real Time System (Bio-Rad) with the iScript TM Green Supermix, cDNA, and custom-designed primers , and the real-time PCR reactions were run in duplicate.

    cDNA Synthesis:

    Article Title: Curcuma longa L. extract exhibits anti-inflammatory and cytoprotective functions in the articular cartilage of monoiodoacetate-injected rats
    Article Snippet: The extracted RNA was assessed for ratio and concentration using a NanoDrop™ spectrophotometer. .. Thereafter, complementary DNA (cDNA) was synthesised from purified total RNA (500 ng/mL) using the iScript TM cDNA Synthesis Kit (Bio-Rad). .. Real-time polymerase chain reaction (PCR) was performed using a CFX Connect TM Real Time System (Bio-Rad) with the iScript TM Green Supermix, cDNA, and custom-designed primers , and the real-time PCR reactions were run in duplicate.

    Article Title: Synthesis and SARs study of novel spiro-oxindoles as potent antiproliferative agents with CDK-2 inhibitory activities.
    Article Snippet: Funding information Researchers Supporting Project, Grant/Award Number: RSP2023R427 Abstract A series of 16 novel spirooxindole analogs 8a–p were designed and constructed via cost‐effective single‐step multicomponent [3+2] cycloaddition reaction of azomethine ylide (AY) generated in situ from substituted isatin (6a–d) with suitable amino acids (7a–c) and ethylene‐engrafted pyrazole derivatives (5a,b).. The potency of all compounds was assayed against a human breast cancer cell line (MCF‐7) and a human liver cell line (HepG2).. Spiro compound 8c was the most active member among the synthesized candidates, with exceptional cytotoxicity against the MCF‐7 and HepG2 cell lines, with IC50 values of 0.189 ± 0.01 and 1.04 ± 0.21 μM, respectively.

    Article Title: Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome.
    Article Snippet: Total RNA was collected using the RNA Clean and Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions. .. RNA was isolated from cultures of CEA17ΔakuBKU80, pabA-IRT, and pksP-IRT with and without doxycycline induction after 24 h. Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA synthesis kit protocol (Bio-Rad Laboratories, Inc.), and the concentration was determined using a Qubit Flex Fluorometer (Thermo Fisher Scientific). ..

    Synthesized:

    Article Title: Synthesis and SARs study of novel spiro-oxindoles as potent antiproliferative agents with CDK-2 inhibitory activities.
    Article Snippet: Funding information Researchers Supporting Project, Grant/Award Number: RSP2023R427 Abstract A series of 16 novel spirooxindole analogs 8a–p were designed and constructed via cost‐effective single‐step multicomponent [3+2] cycloaddition reaction of azomethine ylide (AY) generated in situ from substituted isatin (6a–d) with suitable amino acids (7a–c) and ethylene‐engrafted pyrazole derivatives (5a,b).. The potency of all compounds was assayed against a human breast cancer cell line (MCF‐7) and a human liver cell line (HepG2).. Spiro compound 8c was the most active member among the synthesized candidates, with exceptional cytotoxicity against the MCF‐7 and HepG2 cell lines, with IC50 values of 0.189 ± 0.01 and 1.04 ± 0.21 μM, respectively.

    Article Title: Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome.
    Article Snippet: Total RNA was collected using the RNA Clean and Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions. .. RNA was isolated from cultures of CEA17ΔakuBKU80, pabA-IRT, and pksP-IRT with and without doxycycline induction after 24 h. Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA synthesis kit protocol (Bio-Rad Laboratories, Inc.), and the concentration was determined using a Qubit Flex Fluorometer (Thermo Fisher Scientific). ..

    Article Title: The Type IV Secretion System (T4SS) Mediates Symbiosis between Bradyrhizobium sp. SUTN9-2 and Legumes.
    Article Snippet: Total RNA was treated with RNase-free DNase I (NEB, USA) for 30 min at 37°C. .. Complementary DNA (cDNA) was synthesized from 500 ng of total RNA using iScript reverse transcription Supermix (Bio–Rad, USA) according to the manufacturers’ protocols. ..

    Isolation:

    Article Title: Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome.
    Article Snippet: Total RNA was collected using the RNA Clean and Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions. .. RNA was isolated from cultures of CEA17ΔakuBKU80, pabA-IRT, and pksP-IRT with and without doxycycline induction after 24 h. Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA synthesis kit protocol (Bio-Rad Laboratories, Inc.), and the concentration was determined using a Qubit Flex Fluorometer (Thermo Fisher Scientific). ..

    Concentration Assay:

    Article Title: Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome.
    Article Snippet: Total RNA was collected using the RNA Clean and Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions. .. RNA was isolated from cultures of CEA17ΔakuBKU80, pabA-IRT, and pksP-IRT with and without doxycycline induction after 24 h. Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA synthesis kit protocol (Bio-Rad Laboratories, Inc.), and the concentration was determined using a Qubit Flex Fluorometer (Thermo Fisher Scientific). ..

    Reverse Transcription:

    Article Title: The Type IV Secretion System (T4SS) Mediates Symbiosis between Bradyrhizobium sp. SUTN9-2 and Legumes.
    Article Snippet: Total RNA was treated with RNase-free DNase I (NEB, USA) for 30 min at 37°C. .. Complementary DNA (cDNA) was synthesized from 500 ng of total RNA using iScript reverse transcription Supermix (Bio–Rad, USA) according to the manufacturers’ protocols. ..



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